In our research on role of antigen 43 on biofilm formation in non-157 shiga toxin producing E. coli we found interesting results

The paper title is as follow Role of antigen-43 on biofilm formation and horizontal antibiotic resistance gene transfer in non-O157 Shiga toxin producing Escherichia coli strains

1
0
Upvote 1 Comment

Background and Objectives: The objectives of this study were to evaluate the antibiotic resistance profiles, biofilm formation,
presence of antigen 43 (Ag43) gene, and transfer of antibiotic resistance phenotype among non-O157 Shiga toxin producing Escherichia coli (STEC).
Materials and Methods: From October 2014 to November 2015 a total of 671 stool samples were collected from healthy calves, goats and 395 patients with the sign of nonbloody diarrhea and screened for presence of stx and serotype O157 genes by polymerase chain reaction (PCR) technique. Susceptibility to 14 antibiotics was determined as per CLSI guideline. Presence of Ag43 and intimin (eaeA) genes were detected by PCR. Biofilm formation was measured by microtiter plate method. Conjugation was carried out by membrane filter technique.
Results: We isolated 74 (93.6%) non-O157 STEC strains from 41 calves, 33 goats and 5 (6.3%) patients’ stools, however, no O157 serotype was detected in our study. Resistance was observed most commonly to tobramycin (66.2%), kanamycin (48.6%), and amikacin (29.7%) and less frequently to ciprofloxacin (4.1%), amoxicillin-clavulanic acid (5.4%), and ceftriaxone (9.5%) in isolates recovered from calves and goats fecal samples, whereas, all human isolates were sensitive to ceftazidime, ciprofloxacin, tobramycin and imipenem, respectively. Furthermore, Ag43 was detected in 60 STEC isolated from animals and 5 human origins (no eaeA gene was found in this study). Biofilm formation from Ag43+ and Ag43- colonies showed 20 isolates with strong biofilm activities. Cefotaxime resistance phenotype was transferred to E. coli ATCC 25922.1 (Nalr) by conjugation at a frequency of 1.6×10-4.
Conclusion: From the above results we concluded that, human infections with non-O157 STEC were significantly low in Kerman. Ag43 was insignificant with biofilm quantity in most cases.

Go to the profile of Mohammad reza Shakibaie

Mohammad reza Shakibaie

Associate Professor and senior research scientist at kerman university of medical sciences, Kerman University of Medical Sciences

M.R. Shakibaie earned his PhD from University of Pune India from 1992 to 1997. His thesis was lecular genetics of Plasmid Mediated Silver and Antibiotic Resistances in Acinetobacter baumannii”. He completed one year Postdoctoral research training at Aquatic Microbial Lab in Mysore, India during 2006. His research title was Horizontal Gene Transfer in Gram Negative Bacteria. Dr. Shakibaie Joined the Department of Microbiology, Kerman University of Medical Sciences in 1998. He has published more than 45 original research papers in different International peer reviewed journals with high impact factors in microbiology and biotechnology. The total impact factor of his research is more than 68, with h-index 11 and i10-index 12. His work is cited more than 296 times (www.scholar.google.com). The research gate score index is 21.85. His work also has been cited in 3 textbooks of microbiology published from USA and Europe. He has presented more than 42 papers in International and National Conferences. He has made fundamental contributions in the area of gene transfer, plasmid biology, molecular microbiology, nanomicrobiology. He is on the editorial board of number of peer reviewed journals in Iran and abroad. He has successfully supervised 2 Ph.D., and 1 Post-Doctoral scholars for their research. Currently 2 Ph.D. students are working with him.

Please sign in or register for FREE to comment