Is biofilm associated protein (bap) gene expression influence by Iron concentrations ?

There is no information so far on bap gene expression influences by iron concentration in Acinetobacter baumannii. In our research which carried out by my PhD student for three years, we found that in low iron expression of bap gene was increased four fold by relative quantitative RT-PCR. The paper has been published in Reports of Biochemistry & Molecular Biology 2016; 5(1):62-71.

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There is interesting to now for nature microbial community that the iron may control the amount of biofilm formation. One factor that effect the quantity of biofilm is expression of bap gene. in our research we sequenced bap gene from Acinetobacter baumannii and deposited in the GenBank database (accession no. KR080550.1). Expression of bap in the presence of low iron was analyzed by relative quantitative real time PCR (rqRT-PCR). Nearly half of the isolates belonged to RAPD-types A and B remaining were either small clusters or singleton. The results of biofilm formation revealed that 23 (35.4%), 18 (27.7%), 13 (20%), and 11 (16.9%) of the isolates had strong, moderate, weak, and no biofilm activities, respectively. ompA and csuE genes were detected in all, while bap and blaPER-1 were detected in 43 (66%) and 42 (64%) of the isolates that showed strong and moderate biofilm activities (p ≤ 0.05), respectively. Analysis of bap expression by rqRT-PCR revealed five isolates with fourfold bap overexpression in the presence of low iron concentration (20 µM).

Mohammad Reza Shakibaie

Professor and senior research scientist in microbiology, Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran

I earned my Ph.D. from the University of Pune India from 1992 to 1997. His thesis was "Molecular genetics of plasmid-mediated silver and antibiotic resistances in Acinetobacter baumannii”. I completed one year of postdoctoral research training at Aquatic Microbial Lab in Mysore, India in 2006. The title of my Postdoc research was "Horizontal Gene Transfer in Gram-Negative Bacteria". I joined the Department of Microbiology, Kerman University of Medical Sciences in 1998. I published more than 45 original research papers in different International peer-reviewed journals with high impact factors in microbiology and biotechnology. The total impact factor of his research is more than 86, with h-index 13 and i10-index 27. The research gate score index is 27.85. My works also have been cited in 3 textbooks of microbiology published from the USA and Europe. I presented more than 42 papers at International and National Conferences. He has made fundamental contributions in the area of gene transfer, plasmid biology, molecular microbiology, nanotechnology.  I successfully supervised 4 Ph.D. and 1 Post-Doctoral scholar for their research. Currently, 2 Ph.D. students are working with me. Recently, we published a paper in Iran Biomed J 2021 May 1;25(3):193-201. DOI: 10.29252/ibj.25.3.157 entitled Seterochemical Trajectories of PmrA/B Two-component regulatory system in Colistin Resistant Clinical isolates of Acinetobacter baumannii