Is biofilm associated protein (bap) gene expression influence by Iron concentrations ?
There is no information so far on bap gene expression influences by iron concentration in Acinetobacter baumannii. In our research which carried out by my PhD student for three years, we found that in low iron expression of bap gene was increased four fold by relative quantitative RT-PCR. The paper has been published in Reports of Biochemistry & Molecular Biology 2016; 5(1):62-71.
There is interesting to now for nature microbial community that the iron may control the amount of biofilm formation. One factor that effect the quantity of biofilm is expression of bap gene. in our research we sequenced bap gene from Acinetobacter baumannii and deposited in the GenBank database (accession no.
KR080550.1). Expression of bap in the presence of low iron was analyzed by relative quantitative real time PCR
(rqRT-PCR). Nearly half of the isolates belonged to RAPD-types A and B remaining were either small clusters or
singleton. The results of biofilm formation revealed that 23 (35.4%), 18 (27.7%), 13 (20%), and 11 (16.9%) of the
isolates had strong, moderate, weak, and no biofilm activities, respectively. ompA and csuE genes were detected in
all, while bap and blaPER-1 were detected in 43 (66%) and 42 (64%) of the isolates that showed strong and moderate
biofilm activities (p ≤ 0.05), respectively. Analysis of bap expression by rqRT-PCR revealed five isolates with fourfold
bap overexpression in the presence of low iron concentration (20 µM).