Behind the paper

Recent study by Dr. Shakibaie M.R and co-workers revealed that at low iron concenteration the expression of biofilm associated protein (bap ) gene increases four fold

The paper was published in Rep Biochem Mol Biol. 2016 Oct; 5(1): 62–72. PMCID: PMC5214686

Go to the profile of Mohammad reza Shakibaie
Mohammad reza Shakibaie
Jan 21, 2017
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The sequence of bap was determined and deposited in the GenBank database (accession no. KR080550.1). Expression of bap in the presence of low iron was analyzed by relative quantitative real time PCR (rqRT­PCR). Nearly half of the isolates belonged to RAPD­types A and B remaining were either small clusters or singleton. The results of biofilm formation revealed that 23 (35.4%), 18 (27.7%), 13 (20%), and 11 (16.9%) of the isolates had strong, moderate, weak, and no biofilm activities, respectively. ompA and csuE genes were detected in all, while bap and bla were detected in 43 (66%) and 42 (64%) of the isolates that showed strong and moderate biofilm activities (p ≤ 0.05), respectively. Analysis of bap expression by rqRT­PCR revealed five isolates with four­fold bap overexpression in the presence of low iron concentration (20 µM).

Go to the profile of Mohammad reza Shakibaie

Mohammad reza Shakibaie

Associate Professor and senior research scientist at kerman university of medical sciences, Kerman University of Medical Sciences

M.R. Shakibaie earned his PhD from University of Pune India from 1992 to 1997. His thesis was lecular genetics of Plasmid Mediated Silver and Antibiotic Resistances in Acinetobacter baumannii”. He completed one year Postdoctoral research training at Aquatic Microbial Lab in Mysore, India during 2006. His research title was Horizontal Gene Transfer in Gram Negative Bacteria. Dr. Shakibaie Joined the Department of Microbiology, Kerman University of Medical Sciences in 1998. He has published more than 45 original research papers in different International peer reviewed journals with high impact factors in microbiology and biotechnology. The total impact factor of his research is more than 68, with h-index 11 and i10-index 12. His work is cited more than 296 times (www.scholar.google.com). The research gate score index is 21.85. His work also has been cited in 3 textbooks of microbiology published from USA and Europe. He has presented more than 42 papers in International and National Conferences. He has made fundamental contributions in the area of gene transfer, plasmid biology, molecular microbiology, nanomicrobiology. He is on the editorial board of number of peer reviewed journals in Iran and abroad. He has successfully supervised 2 Ph.D., and 1 Post-Doctoral scholars for their research. Currently 2 Ph.D. students are working with him.

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