In our research on role of antigen 43 on biofilm formation in non-157 shiga toxin producing E. coli we found interesting results

The paper title is as follow Role of antigen-43 on biofilm formation and horizontal antibiotic resistance gene transfer in non-O157 Shiga toxin producing Escherichia coli strains

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Background and Objectives: The objectives of this study were to evaluate the antibiotic resistance profiles, biofilm formation,
presence of antigen 43 (Ag43) gene, and transfer of antibiotic resistance phenotype among non-O157 Shiga toxin producing Escherichia coli (STEC).
Materials and Methods: From October 2014 to November 2015 a total of 671 stool samples were collected from healthy calves, goats and 395 patients with the sign of nonbloody diarrhea and screened for presence of stx and serotype O157 genes by polymerase chain reaction (PCR) technique. Susceptibility to 14 antibiotics was determined as per CLSI guideline. Presence of Ag43 and intimin (eaeA) genes were detected by PCR. Biofilm formation was measured by microtiter plate method. Conjugation was carried out by membrane filter technique.
Results: We isolated 74 (93.6%) non-O157 STEC strains from 41 calves, 33 goats and 5 (6.3%) patients’ stools, however, no O157 serotype was detected in our study. Resistance was observed most commonly to tobramycin (66.2%), kanamycin (48.6%), and amikacin (29.7%) and less frequently to ciprofloxacin (4.1%), amoxicillin-clavulanic acid (5.4%), and ceftriaxone (9.5%) in isolates recovered from calves and goats fecal samples, whereas, all human isolates were sensitive to ceftazidime, ciprofloxacin, tobramycin and imipenem, respectively. Furthermore, Ag43 was detected in 60 STEC isolated from animals and 5 human origins (no eaeA gene was found in this study). Biofilm formation from Ag43+ and Ag43- colonies showed 20 isolates with strong biofilm activities. Cefotaxime resistance phenotype was transferred to E. coli ATCC 25922.1 (Nalr) by conjugation at a frequency of 1.6×10-4.
Conclusion: From the above results we concluded that, human infections with non-O157 STEC were significantly low in Kerman. Ag43 was insignificant with biofilm quantity in most cases.

Mohammad Reza Shakibaie

Professor and senior research scientist in microbiology, Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran

I earned my Ph.D. from the University of Pune India from 1992 to 1997. His thesis was "Molecular genetics of plasmid-mediated silver and antibiotic resistances in Acinetobacter baumannii”. I completed one year of postdoctoral research training at Aquatic Microbial Lab in Mysore, India in 2006. The title of my Postdoc research was "Horizontal Gene Transfer in Gram-Negative Bacteria". I joined the Department of Microbiology, Kerman University of Medical Sciences in 1998. I published more than 45 original research papers in different International peer-reviewed journals with high impact factors in microbiology and biotechnology. The total impact factor of his research is more than 86, with h-index 13 and i10-index 27. The research gate score index is 27.85. My works also have been cited in 3 textbooks of microbiology published from the USA and Europe. I presented more than 42 papers at International and National Conferences. He has made fundamental contributions in the area of gene transfer, plasmid biology, molecular microbiology, nanotechnology.  I successfully supervised 4 Ph.D. and 1 Post-Doctoral scholar for their research. Currently, 2 Ph.D. students are working with me. Recently, we published a paper in Iran Biomed J 2021 May 1;25(3):193-201. DOI: 10.29252/ibj.25.3.157 entitled Seterochemical Trajectories of PmrA/B Two-component regulatory system in Colistin Resistant Clinical isolates of Acinetobacter baumannii