Absorbance "a tool to measure Growth"
The tool to measure bacterial growth is absorbance (600nm). I am amazed to measure different growth phases of bacterial growth by absorbance.
Can anyone tell me what are the components in broth are responsible for absorbance that we measure during bacterial growth. Is it the micro molecules like amino acids (a.a), nucleic acids, vitamins which are absorbing light (knowing that a.a & nucleic acid Abs. max is @ UV) OR as a whole bacterial cell membranes & other components responsible for showing particular absorbance?